| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 30-Jan-2000 |  Jordan Stewart | Leicester City | England | 03-Mar-1982 | 17(333) |
| 13-Dec-1999 |  Adam Nowland | Blackpool | England | 06-Jul-1981 | 18(160) |
| 20-Feb-2000 |  John Terry | Chelsea | England | 07-Dec-1980 | 19(74) |
| 20-May-2000 |  Gareth Barry | Aston Villa | England | 23-Feb-1981 | 19(86) |
| 20-Feb-2000 |  Nyron Nosworthy | Gillingham | Jamaica | 11-Oct-1980 | 19(131) |
| 12-Dec-1999 |  Steven Gerrard | Liverpool | England | 30-May-1980 | 19(195) |
| 12-Dec-1999 |  Danny Schofield | Huddersfield Town | England | 10-Apr-1980 | 19(245) |
| 12-Dec-1999 | Michael Owen | Liverpool | England | 14-Dec-1979 | 19(363) |
| 10-Jan-2000 |  David Dunn | Blackburn Rovers | England | 27-Dec-1979 | 20(14) |
| 13-Dec-1999 | Clarke Carlisle | Blackpool | England | 14-Oct-1979 | 20(60) |
| 30-Jan-2000 | Jon Harley | Chelsea | England | 26-Sep-1979 | 20(126) |
| 09-Apr-2000 |  Aaron Hughes | Newcastle United | Northern Ireland | 08-Nov-1979 | 20(153) |
| 10-Jan-2000 | Damien Duff | Blackburn Rovers | Republic of Ireland | 02-Mar-1979 | 20(314) |
| 12-Dec-1999 | Jon Newby | Liverpool | England | 28-Nov-1978 | 21(14) |
| 10-Jan-2000 | Damien Johnson | Blackburn Rovers | Northern Ireland | 18-Nov-1978 | 21(53) |
| 20-Feb-2000 | Jody Morris | Chelsea | England | 22-Dec-1978 | 21(60) |
| 20-Feb-2000 | Roland Edge | Gillingham | England | 25-Nov-1978 | 21(87) |
| 09-Apr-2000 | Kieron Dyer | Newcastle United | England | 29-Dec-1978 | 21(102) |
| 09-Jan-2000 | Stefan Oakes | Leicester City | England | 06-Sep-1978 | 21(125) |
| 20-Feb-2000 | Neil Clement | Chelsea | England | 03-Oct-1978 | 21(140) |
| 13-Dec-1999 | John Hills | Blackpool | England | 21-Apr-1978 | 21(236) |
| 20-May-2000 | Celestine Babayaro | Chelsea | Nigeria | 29-Aug-1978 | 21(265) |
| 12-Dec-1999 | Jamie Carragher | Liverpool | England | 28-Jan-1978 | 21(318) |
| 09-Jan-2000 | Emile Heskey | Leicester City | England | 11-Jan-1978 | 21(363) |
| 09-Jan-2000 | Stuart Campbell | Leicester City | Scotland | 09-Dec-1977 | 22(31) |
| 13-Dec-1999 |  Thierry Henry | Arsenal | France | 17-Aug-1977 | 22(118) |
| 10-Jan-2000 | David Thompson | Liverpool | England | 12-Sep-1977 | 22(120) |
| 13-Dec-1999 | Alex Manninger | Arsenal | Austria | 04-Jun-1977 | 22(192) |
| 13-Dec-1999 | Fredrik Ljungberg | Arsenal | Sweden | 16-Apr-1977 | 22(241) |
| 12-Dec-1999 | Danny Murphy | Liverpool | England | 18-Mar-1977 | 22(269) |
| 20-May-2000 | Lee Hendrie | Aston Villa | England | 18-May-1977 | 23(2) |
| 13-Dec-1999 | John Murphy | Blackpool | England | 18-Oct-1976 | 23(55) |
| 13-Dec-1999 | Nwankwo Kanu | Arsenal | Nigeria | 01-Aug-1976 | 23(133) |
| 12-Dec-1999 | Rigobert Song | Liverpool | Cameroon | 01-Jul-1976 | 23(163) |
| 20-May-2000 | Mario Melchiot | Chelsea | Netherlands | 04-Nov-1976 | 23(197) |
| 10-Jan-2000 | Callum Davidson | Blackburn Rovers | Scotland | 25-Jun-1976 | 23(198) |
| 09-Jan-2000 | Patrick Vieira | Arsenal | France | 23-Jun-1976 | 23(199) |
| 09-Apr-2000 | Shay Given | Newcastle United | Republic of Ireland | 20-Apr-1976 | 23(354) |
| 20-May-2000 | Mark Delaney | Aston Villa | Wales | 13-May-1976 | 24(7) |
| 12-Dec-1999 | Jamie Vincent | Huddersfield Town | England | 18-Jun-1975 | 24(177) |
| 12-Dec-1999 | Craig Armstrong | Huddersfield Town | England | 23-May-1975 | 24(203) |
| 09-Jan-2000 | Darren Eadie | Leicester City | England | 10-Jun-1975 | 24(213) |
| 12-Dec-1999 | Ben Thornley | Huddersfield Town | England | 21-Apr-1975 | 24(235) |
| 20-Feb-2000 | Andy Hessenthaler | Gillingham | England | 17-Jun-1975 | 24(248) |
| 20-May-2000 | George Boateng | Aston Villa | Netherlands | 05-Sep-1975 | 24(258) |
| 10-Jan-2000 | Keith Gillespie | Blackburn Rovers | Northern Ireland | 18-Feb-1975 | 24(326) |
| 12-Dec-1999 | Sander Westerveld | Liverpool | Netherlands | 23-Oct-1974 | 25(50) |
| 09-Jan-2000 | Robbie Savage | Leicester City | Wales | 18-Oct-1974 | 25(83) |
| 09-Apr-2000 | Nolberto Solano | Newcastle United | Peru | 12-Dec-1974 | 25(119) |
| 13-Dec-1999 | Ian Hughes | Blackpool | Wales | 02-Aug-1974 | 25(133) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).