| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 18-Jun-2020 |  Kwame Poku | Colchester United | England | 11-Aug-2001 | 18(312) |
| 18-Jun-2020 |  James Olayinka | Northampton Town | England | 05-Oct-2000 | 19(256) |
| 18-Jun-2020 |  Jack Sparkes | Exeter City | England | 29-Sep-2000 | 19(262) |
| 22-Jun-2020 | Jayden Richardson | Exeter City | England | 04-Sep-2000 | 19(291) |
| 18-Jun-2020 | Callum Morton | Northampton Town | England | 19-Jan-2000 | 20(151) |
| 18-Jun-2020 | Archie Collins | Exeter City | England | 31-Aug-1999 | 20(292) |
| 22-Jun-2020 | Ben Seymour | Exeter City | England | 16-Apr-1999 | 21(68) |
| 18-Jun-2020 |  Jake Doyle-Hayes | Cheltenham Town | Republic of Ireland | 30-Dec-1998 | 21(171) |
| 18-Jun-2020 | Scott Wharton | Northampton Town | England | 03-Oct-1997 | 22(259) |
| 18-Jun-2020 | Courtney Senior | Colchester United | England | 30-Jun-1997 | 22(354) |
| 18-Jun-2020 | Ben Stevenson | Colchester United | England | 23-Mar-1997 | 23(87) |
| 18-Jun-2020 | Lewis Ward | Exeter City | England | 05-Mar-1997 | 23(105) |
| 18-Jun-2020 | Randell Williams | Exeter City | England | 30-Dec-1996 | 23(170) |
| 18-Jun-2020 | Owen Evans | Cheltenham Town | Wales | 28-Nov-1996 | 23(202) |
| 22-Jun-2020 | Miles Welch-Hayes | Colchester United | England | 25-Oct-1996 | 23(240) |
| 18-Jun-2020 | Ryan Broom | Cheltenham Town | Wales | 04-Sep-1996 | 23(287) |
| 18-Jun-2020 | Cohen Bramall | Colchester United | England | 02-Apr-1996 | 24(77) |
| 18-Jun-2020 | William Boyle | Cheltenham Town | Scotland | 01-Sep-1995 | 24(291) |
| 18-Jun-2020 | Charlie Goode | Northampton Town | England | 03-Aug-1995 | 24(320) |
| 18-Jun-2020 | Harry Smith | Northampton Town | England | 18-May-1995 | 25(32) |
| 18-Jun-2020 | Tom Lapslie | Colchester United | England | 05-May-1995 | 25(45) |
| 18-Jun-2020 | Sean Long | Cheltenham Town | Republic of Ireland | 02-May-1995 | 25(48) |
| 18-Jun-2020 | Jordan Turnbull | Northampton Town | England | 31-Oct-1994 | 25(231) |
| 18-Jun-2020 | Pierce Sweeney | Exeter City | Republic of Ireland | 11-Sep-1994 | 25(281) |
| 18-Jun-2020 | Jonte Smith | Cheltenham Town | Bermuda | 10-Jul-1994 | 25(344) |
| 22-Jun-2020 | Alex Addai | Cheltenham Town | England | 20-Dec-1993 | 26(185) |
| 18-Jun-2020 | Conor Thomas | Cheltenham Town | England | 29-Oct-1993 | 26(233) |
| 22-Jun-2020 | Jonathan Maxted | Exeter City | England | 26-Oct-1993 | 26(240) |
| 18-Jun-2020 | Brennan Dickenson | Exeter City | England | 09-Sep-1993 | 26(283) |
| 18-Jun-2020 | Tom Nichols | Cheltenham Town | England | 28-Aug-1993 | 26(295) |
| 18-Jun-2020 | Ryan Watson | Northampton Town | England | 07-Jul-1993 | 26(347) |
| 18-Jun-2020 | Alfie May | Cheltenham Town | England | 02-Jul-1993 | 26(352) |
| 18-Jun-2020 | Luke Norris | Colchester United | England | 03-Jun-1993 | 27(15) |
| 18-Jun-2020 | Charlie Raglan | Cheltenham Town | England | 28-Apr-1993 | 27(51) |
| 18-Jun-2020 | Luke Gambin | Colchester United | Malta | 16-Mar-1993 | 27(94) |
| 22-Jun-2020 | Sam Hoskins | Northampton Town | England | 04-Feb-1993 | 27(138) |
| 18-Jun-2020 | Rohan Ince | Cheltenham Town | England | 08-Nov-1992 | 27(222) |
| 18-Jun-2020 | Michael Harriman | Northampton Town | Republic of Ireland | 23-Oct-1992 | 27(238) |
| 18-Jun-2020 | Tom Parkes | Exeter City | England | 15-Jan-1992 | 28(155) |
| 18-Jun-2020 | Jake Taylor | Exeter City | Wales | 01-Dec-1991 | 28(200) |
| 18-Jun-2020 | Ryan Bowman | Exeter City | England | 30-Nov-1991 | 28(201) |
| 18-Jun-2020 | Vadaine Oliver | Northampton Town | England | 21-Oct-1991 | 28(241) |
| 18-Jun-2020 | Tom Eastman | Colchester United | England | 21-Oct-1991 | 28(241) |
| 18-Jun-2020 | Harry Pell | Colchester United | England | 21-Oct-1991 | 28(241) |
| 18-Jun-2020 | Frank Nouble | Colchester United | England | 24-Sep-1991 | 28(268) |
| 18-Jun-2020 | Paris Cowan-Hall | Colchester United | England | 05-Oct-1990 | 29(257) |
| 18-Jun-2020 | Ryan Jackson | Colchester United | England | 31-Jul-1990 | 29(323) |
| 22-Jun-2020 | Alex Fisher | Exeter City | England | 30-Jun-1990 | 29(358) |
| 18-Jun-2020 | Ben Tozer | Cheltenham Town | England | 01-Mar-1990 | 30(110) |
| 18-Jun-2020 | Aaron Martin | Exeter City | England | 29-Sep-1989 | 30(263) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).